SHUISKY V.F., MAXIMOVA T.V., SOLOVEY N.A., SANOTSKAYA N.A.
Ltd. "Eco-Express-Service", Russia

The report contains the theoretical basis and description of evaluation and standard-setting quantitative method of complex (multifactor) impacts on waters ecosystems. The suggested method uses the revealed general laws of the best hydroecosystem bioindicator reaction – macrozoobenthocoenoses – to multicomponent external impact, which is estimated by the original isobolic index. The present techniques and data are based on representative long-term hydroecological research results executed by the group of authors. Basic methodological positions and practical applications of this method are illustrated in detail by the factual data. Particular examples of the method used for solving various environmental protection problems are also considered.

1. Problem of quantitative evaluation of multifactor anthropogenic impacts on hydroecosystems
The comparative analysis of various methods of hydroecosystems state and its change evaluation is realized.

The following positions are proved:
– The quantitative evaluation of multifactor impacts should be carried out on the universal index development base.
– This index should correspond to the following requirements:
- to be isobolic (its concrete value should correspond to all multifactor impact isoboles and determine reciprocal reaction of biota);
- to reflect adequately and exactly the effect of every combination of interacting factors;
- to express the rate of excess by the current impact level to its maximum permissible level (i.e. to carry out standard-setting of current impact).
– Anthropogenic successions of hydroecosystem should be evaluated on the basis of the changes caused to the most indicative bioindicator – macrozoobenthos state.
– The major macrozoobenthos characteristic is its species composition. Accordingly, the maximum permissible level of anthropogenic impact is the its greatest impact level, which does not cause changes of macrozoobenthos species composition. Anthropogenic changes of quantitative structural and functional macrozoobenthos characteristics also are important for bioindication, but they are secondary, auxiliary.

The importance is proved of establishing and describing the basic quantitative laws of macrozoobenthos species composition and its structural functional characteristics reaction on the multifactor anthropogenic impacts, expressed by isobolic index.

The report generalizes some of the results of a long-term study of anthropogenic succession of polytypic aquatic objects ecosystems in Russia and in Finland. The used database includes the results of processing more than six thousand macrozoobenthos samples and more than one thousand water and bottom samples.

2. Macrozoobenthocoenosis resistance to multifactor impact

The concept of evaluation of macrozoobenthocoenoses resistance to external impact is explained. The criterion is introduced of allocation of those species, which should be regarded as characteristic for macrozoobenthocoenosis during its study and taken into account during a comparative evaluation of species structure exogenic changes. Concepts and attributes of natural habitat conditions and macrozoobenthocoenosis background state are justified. The procedure of describing macrozoobenthocoenosis background state in polytypic habitats is defined. The basic components of the concept and a method of the macrozoobenthocoenosis background condition description, species structure and bioindicatelly significant quantitative characteristics of the coenosis are in detail illustrated through sample ecosystems.

The concrete concept and quantitative measure of macrozoobenthocoenosis elastic resistance ("elasticity") to external impact are suggested. Elasticity is treated as an ability of the coenosis to come back to natural condition after impact discontinuance, determined by preservation of species structure, characteristic for this coenosis in its natural state. Accordingly, a proved change of characteristic species structure testifies that the coenosis has lost its elasticity.

The suggested concept allows us to move on to the quantitative description of elasticity saving conditions.

3. Quantitative description of macrozoobenthocoenosis resistance preservation conditions in the case of multifactor impact
The system of the interconnected parameters is justified and introduced, which are necessary for the quantitative description of macrozoobenthocoenosis resistance preservation condition in the case of external impact. Methodology and method of the quantitative description of coenosis elasticity are introduced and explained gradually, in process of solved problems complication. At first, the elementary case of one factor action, then - a combination of two factors and, at last, the situation of infinitely complex multifactor interaction are considered one after another. Some results of a quantitative evaluation of macrozoobenthocoenosis elastic resistance at various hydroecosystems, studied by authors are generalized. The maximum permissible level of external impact on the indicator - macrozoobenthos and the ecosystem it represents is described in factor hyperspace as the boundary of the allowed factor value combination area.

All stages of the quantitative description of macrozoobenthocoenosis resistance preservation condition in the case of multifactor impact are shown in detail through particular examples.

Quantitative description of macrozoobenthocoenosis elasticity preservation condition allows us to consider a solution of the following problem: measurement of multifactor impact by means of comparing its current level with the appropriate maximum permissible level.

4. Quantitative evaluation and standard-setting of multifactor anthropogenic impact on macrozoobenthocoenoses
The universal index of resulting multifactor impact level, witch is appropriate to all necessary criteria is suggested (chapter 1). This index expresses the rate of excess by impact of macrozoobenthos elastic resistance. The all-round approbation of this index is realized:
- the index is really isobolic and it determines changes of major characteristics of biota;
- the index takes into account adequately and flexibly complex factor interaction effect in any combination of this factors;
- the index is dimensionless and it expresses the rate of excess by any complex impact of its maximum permissible level, determined by macrozoobenthocoenosis elastic resistance in the case of this impact.

The suggested isobolic index of multifactor impact has besides some additional advantages. If the impact has not yet caused any loss of macrozoobenthocoenosis elasticity, the index also characterizes "safety factor" of the coenosis (its residual resistance). This index gives besides exact evaluation of the each factor contribution to their common man-made burden on biota.

Use of the isobolic index allows to analyze, generalize, formalize and typify the basic quantitative laws of macrozoobenthocoenosis reaction on various multifactor impacts. The uniform classification and the approach to standard-setting of multifactor anthropogenic impacts on hydroecosystems are suggested.

The evaluation of various complex impacts and different variants of anthropogenic macrozoobenthocoenosis changes are illustrated in details through particular examples.

5. Some examples of isobolic method use
Examples of isobolic method practical application are presented to solve the following environmental and nature protection problems:
- the studying and the evaluation of multifactor anthropogenic impacts on various aquatic ecosystems;
- the analysis of environmental risk and the definition of environmental damage for aquatic objects in the case of multifactor anthropogenic impact;
- the proved choice of effective measures on technogenic impact regulation and the expected result predictions.
The target audience of the present book are specialists in the field of hydroecology, environmental safety, ecological toxicology and zoology as a broader audience of the readers interested in problems of studying external impacts on biosystems or other system objects.